In present study, a successful attempt has been made to develop a simple method for the simultaneous determination of Gatifloxacin (GATI) and Ornidazole (ORNI) using UV spectrometric technique. UV Spectroscopic method which is based on measurement of absorption of UV light, the spectra of Gatifloxacin and Ornidazole in methanol : water (90:10) showed maximum wave length at 292nm and 313nm respectively and calibration graphs were plotted over the concentrations ranging from 1-20µg/ml of Gatifloxacin and Ornidazole with correlation coefficient 0.999. Validation was performed as per ICH Q2 (R1) guidelines for linearity, accuracy, precision and recovery. The limit of detection (LOD) and limit of quantification (LOQ) of Gatifloxacin were found to be 0.061 and 0.187 and for Ornidazole limit of detection and Limit of quantification were found to be 0.300, 0.028 respectively by simple UV Spectroscopy. Multicomponent method of analysis was validated by analyzing the laboratory prepared samples. The % mean results were found to be 99.17 for Gatifloxacin & 99.15 for Ornidazole. Similarly the standard deviation of Gatifloxacin & Ornidazole were found to be 0.334 & 0.180 respectively and the analysis of formulation was studied & it was found that the % mean were 99.37 & 99.07 for Gatifloxacin & Ornidazole respectively, where the Standard Deviation were found to be 0.258 & 0.109 respectively which indicate that the developed method is accurate, precise & sensitive.
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